Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • New Method for DNA Sequence Amplification

    Click image for larger version

Name:	Low-Res_MohamedDraz.jpg
Views:	321
Size:	48.6 KB
ID:	325788
    Mohamed S. Draz, leader of the AMPLON study and researcher at Case Western Reserve University. (Image Credit: Case Western Reserve University)


    A research team from Case Western Reserve University School of Medicine has developed an innovative method for target DNA sequence amplification, testing, and analysis. This technique, named AMPLON (Amplifying DNA with Multiarm Priming and Looping Optimization of Nucleic Acid), offers a promising alternative to the traditional Polymerase Chain Reaction (PCR) method, potentially broadening its applications in medical diagnosis.

    "AMPLON has the potential to positively change the way molecular analysis and clinical diagnostics are performed," stated Mohamed S. Draz, an assistant professor at the School of Medicine and the principal investigator of the study, "from infectious-disease diagnostics to personalized medicine and environmental monitoring."

    Mechanism of AMPLON
    AMPLON improves DNA synthesis by providing multiple extensions along the DNA strand, which simultaneously increases the speed and accuracy of the process under constant temperature conditions. This method eliminates the need for the high and low-temperature cycling required by PCR, reducing stress on materials and making the process more accessible, especially in environments where precise temperature control is challenging.

    In traditional PCR, DNA samples are subjected to high temperatures to separate them into single strands. An enzyme then builds two new strands using the originals as templates. This process is labor-intensive, time-consuming, and costly.

    "We’ve developed a new method of DNA amplification that does not require bulky lab-bound equipment but can be conducted in one step and in diverse settings," Draz explained. "More significantly, our approach does not weaken enzymes like the PCR method."

    Advantages and Applications
    AMPLON's multiarmed DNA primer design converts the weaknesses of enzymes into strengths, enhancing amplification efficiency and consistency. This improvement reduces the amplification time by 50%, providing a portable, reliable, and cost-effective solution for nucleic acid amplification.

    "We’ve been able to enhance amplification and reduce amplification time by 50%," Draz noted. "Our approach has the potential to dramatically change the way nucleic acid amplification is performed, providing instead a portable, reliable, and cost-effective solution for applications, ranging from point-of-care diagnostics to field-based research."

    Publication Details
    Doganay, M. T., Roman, E., Hujer, A. M., Bonomo, R. A., Deeks, S. G., Kuritzkes, D. R., & Draz, M. S. (2024). AMPLON: Amplifying DNA with Multi‐Arm Priming and Looping Optimization of Nucleic Acid. Advanced Materials, 2311634. doi:https://doi.org/10.1002/adma.202311634

Latest Articles

Collapse

  • seqadmin
    Best Practices for Single-Cell Sequencing Analysis
    by seqadmin



    While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
    06-06-2024, 07:15 AM
  • seqadmin
    Latest Developments in Precision Medicine
    by seqadmin



    Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

    Somatic Genomics
    “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
    05-24-2024, 01:16 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 07:24 AM
0 responses
10 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-13-2024, 08:58 AM
0 responses
11 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-12-2024, 02:20 PM
0 responses
16 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-07-2024, 06:58 AM
0 responses
184 views
0 likes
Last Post seqadmin  
Working...
X