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fibar · 07-15-2013, 07:16 AM

Our goal: to reconstruct individual genomes from a metagenome
Our method: Illumina Hiseq paired-end sequencing

What would you choose...

LouiseT · 05-28-2013, 06:53 PM

Hi there
I am comparing exome seq data from 3 platforms; Solid4, Solid5500 and HiSeq(Illumina).
I have set the min. depth to 10 but I am...

NicoBxl · 02-26-2013, 12:39 AM

Hi everybody,

I've several questions about sequencing depth and RNA-seq. So, what is the minimal read count (in the raw data) to have a...

litc · 08-29-2012, 12:27 AM

I have got a RAD data set and assessed it. It seems the depth of RAD fragment vary differently, extremely different.

the reads(extract...

bioinf newbie · 02-15-2012, 12:58 PM

How do you calculate the genome coverage for a sequenced genome?
I have the BAM file for my genome, and I have calculated the read depth at each...

schelhorn · 02-14-2012, 08:50 AM

Dear All,

I apologize if an existing answer to the following, basic question is somewhere buried in the forum - if yes, then a quick search...

mblue · 02-12-2012, 05:38 PM

Hi,
I want to do a differential analysis between two conditions and I have 3 biological replicates per condition. All samples have similar number...

melo · 05-10-2011, 07:50 AM

Dear all

I am finding difficult to understand some features of the vcf (variant call format) files I am getting when calling SNPs...

oliviera · 10-15-2010, 04:08 AM

Dear all,

I try to get an idea of the base coverage required for the different sequencing applications.
I didn t find any review...

Anelda · 08-31-2010, 01:00 AM

Hi all,

I have done reference mapping using gsMapper. I want to calculate depth of coverage for various contigs obtained. According to...

arendon · 05-28-2009, 08:42 AM

Hello,

I would like to sequence coverage along the genome from aligned reads. Any suggestions or tools to do this efficiently? Maybe also...

bioinfosm · 09-09-2008, 09:50 AM

> Hi,
>
> From my understanding MAQ is tested for 20-50x coverage regions for SNP
> calling. However, we routinely have...

Depth & Length for individual genome reconstructrion