23 results in 0.0022 seconds.
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Treating extractions with RNase
Hi. We have a collaborator who wishes to prep ddRAD libraries using several hundred DNA extractions, but they have not yet been treated with RNase A.... -
Rare cutter not cutting? (ddRAD)
Greetings, everyone!
I've been testing my restriction enzymes, and have candidates for a rare cutter and a common cutter. The rare cutter... -
Brainstorming "ddRAD-BiS"
Hi there,
I'm a newbie with sequencing, and I'm currently thinking about new ideas for a grant application. I work on non-model organisms... -
ddRAD library low concentration
Greetings,
I am making a ddRAD library and post-digest and cleanup my concentrations are very low. Since I have to standardize my DNA concentrations... -
Problem with uneven distribution of rads among individual libraries
Hello everyone!
I am totally new in NGS, so I apologize for the basic question.
I am doing ddRAD (EcoRI and SphI) for SNP analysis... -
ddRAD size selection with Ampure beads
Hi there,
I am looking at using a modified ddRAD protocol in a genus of plants with a genome approximately 1000Mbp in size. I do not have... -
ddRAD analysis pipeline
Hi,
We are starting to generate ddRAD sequence data (as described by Peterson et al., 2012, http://www.plosone.org/article/info%3Adoi%... -
ddRAD-Seq
Hi all,
I am following the ddRAD-Seq protocol of Peterson et al. 2012. I start with ~40-100 ng/ul DNA as measured on a nanodrop. I take...
