Sample Prep / Library Generation

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Using ethanol as a preservation method for shotgun sequencing by samd199 Started by samd199, 01-16-2023, 08:40 PM	2 responses 36 views 0 likes	Last Post by samd199 01-20-2023, 11:24 AM
RNA fragmentation in presence of heat and Mg++ by cey1001 Started by cey1001, 01-06-2023, 09:12 AM	0 responses 35 views 0 likes	Last Post by cey1001 01-06-2023, 09:12 AM
SPRI Beads & Magnetic Beads: First-hand Tips by BioDynami Started by BioDynami, 01-05-2023, 02:13 PM	0 responses 26 views 0 likes	Last Post by BioDynami 01-05-2023, 02:13 PM
Bioanalyzer problem-rna ladder 600 nano by Arianna Cerana Started by Arianna Cerana, 01-03-2023, 06:11 AM	4 responses 40 views 0 likes	Last Post by Arianna Cerana 01-04-2023, 09:45 AM
Diluting Nextera XT Indexes? by Amygdala Started by Amygdala, 10-15-2020, 08:41 AM	1 response 1,977 views 0 likes	Last Post by kmkocot 12-18-2022, 02:41 PM
Problem with ATACseq Libarary? by ASadiasa Started by ASadiasa, 12-15-2022, 04:43 AM	0 responses 34 views 0 likes	Last Post by ASadiasa 12-15-2022, 04:43 AM
Inefficient ATAC-seq library prep amplification by lapensee Started by lapensee, 11-29-2018, 05:27 AM	22 responses 12,897 views 0 likes	Last Post by ASadiasa 12-15-2022, 04:34 AM
10x 5' GEX - overrepresentation of RT Primer in results by egotistdown Started by egotistdown, 11-15-2021, 07:45 AM	1 response 1,699 views 0 likes	Last Post by xie186 11-22-2022, 06:33 PM
Duplex PCR not working by U17slash Started by U17slash, 11-14-2022, 11:13 PM	0 responses 17 views 0 likes	Last Post by U17slash 11-14-2022, 11:13 PM
TET-assisted pyridine borane sequencing(TAPS) by yizhe2011 Started by yizhe2011, 10-21-2022, 10:48 PM	0 responses 47 views 1 like	Last Post by yizhe2011 10-21-2022, 10:48 PM

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ESPRESSO: Quantifying transcript isoforms from long-read sequencing by seqadmin Started by seqadmin, 01-23-2023, 12:48 PM	0 responses 28 views 0 likes	Last Post by seqadmin 01-23-2023, 12:48 PM
Top 11 Takeaways from JP Morgan Healthcare 2023 by seqadmin Started by seqadmin, 01-18-2023, 04:23 PM	1 response 115 views 0 likes	Last Post by OlgaCaswell 01-25-2023, 05:27 AM
Long-Read Sequencing: Nature’s Method of the Year 2022 by seqadmin Started by seqadmin, 01-13-2023, 03:34 PM	0 responses 33 views 0 likes	Last Post by seqadmin 01-13-2023, 03:34 PM

A Brief Overview and Common Challenges in Single-cell Sequencing Analysis

by seqadmin

The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations¹, cell lineages², cellular diversity and regulation³, and development in multicellular organisms⁴. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i...
- Channel: Articles
01-24-2023, 01:19 PM
Introduction to Single-Cell Sequencing

by seqadmin

Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.

The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes...
- Channel: Articles
01-09-2023, 03:10 PM

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