Hi guys!
I'm trying to run DBG2OLC with contigs built with platanus + 13x times genome coverage in PacBio subreads (fastq). But looks like the...
156 results in 0.0033 seconds.
-
DBG2OLC - parameter f cannot identify my PacBio reads
-
snp calling by Pacbio reads
Hi guys,
Glad to find this place.
Now I'm trying to use 50X pacbio reads for human genome re-sequencing analysis, like... -
GFA to fasta file
Hey guys!
Can someone help me writing a awk line for extracting FASTA sequences from a GFA file? (I lack programming skills and will take... -
Should I try hybrid assembly with my PacBio data?
Hi all,
I recently had the genome of a bacterial strain I am working with sequenced using both PacBio and Illumina paired end.
... -
Fungal Genome Assembly... a painful experience
Hi all and thanks for any insight you have in advance.
I am trying to assemble a fungal genome of approximately 42Mb in size. I have reads... -
Does the full length sequencing using Iso-Seq get the real full length ?
I have used pacbio SMRT Iso-seq module to analyze my cDNA sequencing data, including Getting full length reads, Isoform level clustering (ICE and Quiver),... -
Is There Any Tools/Methods For PacBio IsoSeq New gene prediction analysis?
We have used the SMRT(Pacific Biosciences) sequencing system PacBio RS II to generate cDNA sequences. After isoform-level clustering, polishing and trimming... -
De novo genome assembly strategy
Assembling a genome de novo. I have:
10X coverage with PAC-BIO reads
100X coverage with Illumina short reads (150 bp paired-end... -
PacBio palindromic amplicons
Hi,
We are trying to perform PacBio sequencing on 2-4 kb amplicons that have palindromic sequence. My understanding is that when building... -
Pulling together contigs: a less stringent assembly
Greetings,
New grad student here working with a fungi with a genome that is roughly 42-50Mbs. Basically what I'm trying to do is an assembly... -
file transfer in XSEDE User Portal
Has anyone experience with the XSEDE portal for assembling PacBio reads using the smrtanalysis pipeline? I have found smrtanalysis on a machine called... -
How to run TOFU analysis in the perl ?
Now I am working on the Iso_seq Analysis using the Tofu program, I am intended to intergrated all the analysis (including classify,cluster,Removing Redundant... -
Post-doc Fellow, Third-generation Sequencing & Non-traditional Epigenomics (New York)
In Brief: Epigenomics, PacBio, Oxford Nanopore, RNA Sequencing, Systems/Network biology of infectious diseases and genetic disorders.
Job... -
Any Tools/Workflows For PacBio IsoSeq SSR Analysis?
We have used the SMRT(Pacific Biosciences) sequencing system PacBio RS II to generate cDNA sequences. After isoform-level clustering, polishing and trimming... -
In-dels Illumina vs. PacBio
Hello,
I have a PacBio-generated closed complete bacteria genome. I am aligning Illumina reads of the same strain to it using breseq,...
