Hi,
We are trying to perform PacBio sequencing on 2-4 kb amplicons that have palindromic sequence. My understanding is that when building the circular consensus, the CCS Reads that are palindromic are flagged and discarded since it identifies the reads as misidentification of the adaptor sequence. Since our amplicons are predominantly palindromic, is there a way to silence this feature in the ConsensusTools' code?
Thank you.
We are trying to perform PacBio sequencing on 2-4 kb amplicons that have palindromic sequence. My understanding is that when building the circular consensus, the CCS Reads that are palindromic are flagged and discarded since it identifies the reads as misidentification of the adaptor sequence. Since our amplicons are predominantly palindromic, is there a way to silence this feature in the ConsensusTools' code?
Thank you.
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