156 results in 0.0033 seconds.
-
How can I get the report by using SMRT command-line version?
Now I have RS_IsoSeq command-line installed, and it works well for filter(getting reads of insert) classify(Getting full length reads) ,Isoform level... -
Import raw PacBio data from *.bax.h5 files
Dear all,
I've been sent some raw PacBio data from a collaborator which I'd like to import into SMRT Portal to generate filtered subreads... -
Subreads alignment alternatives
Hi,
Just wondering if anybody has tried to align subreads and build the CCS using alternative alignements programs, others than Blasr.
... -
Identify Translated Sequence in Batch
Does anyone have any suggestions on how to identify in batch for isoseq data the start and stop positions for predicted gene sequence?
... -
Amplicon consensus calling from sub-reads
Hi everyone,
I just got my first PacBio data in and would like some advice on how to handle the data. I submitted a 1KB amplicon library... -
Is There Any Tools/Methods For PacBio IsoSeq SNP/Fusion Genes Analysis?
Hi, there!
I have used pacbio SMRT Iso-seq module to analyze my cDNA sequencing data, including Getting full length reads, Isoform level clustering... -
how to extract alternative splicing information from Iso-seq nonredundant sequence?
I have used pacbio SMRT Iso-seq module to analyze my cDNA sequencing data, including Getting full length reads, Isoform level clustering (ICE and Quiver),... -
PacBio SMRT Informatics Developers Conference – August 26
Hi all,
PacBio will be having a full-day, collaborative conference focused on developing and improving data analysis tools for SMRT Sequencing... -
Start genome at origin
Hello,
I would like to know if there is a simple way/method to start a new genome assembly from PacBio after the circularizing.
... -
PacBio Amplicon reads assembly
I've got a question regarding the assembly of PacBio reads. We've created a library of approximately 5000 different amplicons between 1 and 3kb and successfully... -
Pacbio Large spike at 4kb subread length
Hi all,
I'm running an HGAP on 450kb genome and it's the first time we get this kind of PacBio data. The assembly isn't very good (many... -
Best parameter for bacteria de novo assembly - HGAP
Hello,
I would like to know what is best for bacteria genome assembly in general. I work on 4M~6M genomes in general and they have 1 or 2...Last edited by heyyyjude; 05-23-2015, 08:36 PM. -
smrtpipe.py, HGAP run - detailed description of reports generated
Hello,
I would like to learn few details about the following reports from HGAP run, generated by smrtpipe.py:
...Last edited by Monika_bioinf; 05-14-2015, 01:36 PM. -
LSC-example test error
Hi all, I am trying to use LSC to correct my pacbio reads with illumina reads. However, after installation, I ran into problems running LSC example data.... -
Identifying heteroduplexes in PacBio CCS reads
Hello,
I'm working with circular consensus reads from a PCR product that may contain heteroduplexes. This is because the PCR was generated...Last edited by verheytb; 04-21-2015, 12:45 PM.
