I have used pacbio SMRT Iso-seq module to analyze my cDNA sequencing data, including Getting full length reads, Isoform level clustering (ICE and Quiver), Removing redundant transcripts. So now I have the isoform consensus result. But I don't know how to extract the detail alternative information (including alternative type, alternative start site, alternative end site). I don't know any software or script can do this?
What is the best approach?
Thanks for any comments in advance.
What is the best approach?
Thanks for any comments in advance.
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