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Zimtblatt · 07-11-2021, 04:13 AM

Hello Everyone!

I'm Zimtblatt and I am happy to have found this community.

I'm from a biology background and have been analyzing...

menegidio · 06-04-2019, 05:26 PM

I have a microbiome dataset and I am trying to compare different groups of mice (symptomatic animals, asymptomatic animals and a control group).
...

neko_33 · 12-02-2016, 03:27 AM

Hi SEQ-users,

We've been using the following protocol for 16S V4 region amplicon sequencing on MiSeq, which I think is very familiar to...

rEDI · 09-09-2016, 03:24 AM

Hi

I am having difficulty understanding how my merged reads are producing a certain amplicon size.

Basically, I have 2 x...

maddo1ck · 05-25-2016, 06:59 AM

New member and new to running my own MiSeq Sequencer!

We are trying to figure out how to modify TruSeq PCR free DNA library prep kit protocol...

rEDI · 04-21-2016, 12:40 AM

I am sequencing samples from animal gut using illumina MiSeq/HiSeq, targeting the v4 region of the 16S

I get 22m raw reads back from MiSeq...

Egansbay · 02-25-2016, 10:12 AM

Hi everyone

Probably a stupid question, but i'm finding it hard to find meaningful info on sequencing depth and read numbers on the MiSeq....

manjari.deshmukh · 01-20-2016, 09:38 PM

Dear all,
I am doing a 16S-metagenome project with paired-end reads. I used join_paired_end.py from Qiime. There are around 5000 reads fail to...

bio_informatics · 06-30-2015, 10:46 AM

Hi SeqA team,

I've hit another road block.

Data information: 16s V3-V4 region, demultiplexed. Illumina, Paired end. PhiX 5%...

Egansbay · 03-18-2015, 10:31 AM

Hi everyone

Recently received my sequences back from an Illumina MiSeq 16S V4 300bp PE run, performed by a biotech company. The DNA was...

Egansbay · 01-07-2015, 07:57 AM

Hi all

I'm running a microbiome analysis of fish gut microdlora using Illumina MiSeq 16s pipeline. I'd like to get your thoughts on my...

Egansbay · 01-05-2015, 07:28 AM

Dear all

I am analyzing gut samples from fish using illumina's 16S protocol, amplifying the v4 hypervariable region. I am getting ok bands...

Richa Sharma · 10-29-2014, 02:49 AM

Hi,

I have 16S rRNA datasets produced by Mothur and I want to plot my data and get publictaion quality figures (stacked bars, heatmaps,...

New to 16S Seq on MiSeq