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Amplicon vs Shotgun Metagenomics – How Do I Choose?
Metagenomics is the study of microbial communities in their original habitats and gives a comprehensive insight into the biochemical and metabolic interactions... -
Forward and reverse reads overlap but don't align properly
I am working with Archaea F and R reads acquired with the 344f and 915r primers. Each read is 300bp long so there is overlap but it is only 29 bp. I want... -
16S sequencing from total RNA?
I'm working with a researcher that wants to identify the bacterial communities present in human dental pulp tissue, blood, and saliva from isolated RNA.... -
16S rRNA Data Analysis with Interactive Results by World Fusion
World Fusion offers 16S/18S/ITS metagenomic data analysis services to measure the composition and diversity of microbial or fungal species from sequencing... -
Bioinformatics and cheminformatics tools and services from World Fusion US, Inc.
Introduction:
World Fusion was established in 1996 and has offices in San Diego, CA, and Tokyo, Japan. We have been developing genomic... -
Pathogen and Probiotics database of 16S rRNA
Hi all,
I want to use 16S rRNA reference that has clinical information such as pathogenetic and probiotic information.
What is the... -
Microbiome Analysis Core
Dear All,
I would like to introduce our Microbiome Analysis Core here at the Harvard School of Public Health (HMAC).
Here's... -
Hunter-gatherer microbiome more biodiverse than urban controls
Interesting paper shows that Hadza of Tanzania have higher levels of microbial richness and biodiversity than Italian urban controls. Examines microbial... -
how will manipulating low quality base pairs of reads affect TopHat and Cufflinks?
Hi all,
I am working with a paired-end Illumina 2*250bp reads (16S rRNA). The quality is extremely low (i.e. with quality filtering only... -
surprisingly hard: Going from Genbank Accession number to Genome Name
Hi everybody,
I wrote an automated FASTQ based 16S rrna searcher, so you give it your FASTQ and it tells you which 16S matches it best.... -
Can I combine 16S and 18S rRNA in one sequencing run?
Hello,
I wanted to examine a complex system for the presence of both prokaryotic and eukaryotic microbes.
Is it possible... -
Library prep protocol for 16s rRNA sequencing by MiSeq
Hello everyone,
I'm a PhD student in a lab that just started up and we want to do a microbiome analysis on the gut bacteria in a local... -
MiSeq: V1-2 vs V4 for 16S rRNA amplicon sequencing
Hi guys,
I'm new to this forum and have a question I couldn't find an answer to.
I plan to sequence fish fin samples to... -
demultiplexing data
Hi,
I am totally new to this field, I have a problem while multiplexing the data. I have downloaded the .fastq files for a particular study from... -
400bp chemistry 16s Amplicons
Dear all,
I would like to know if anyone has tried sequencing 16s rDNA amplicons that are in the correct size range (~320bp) using the...
