Hi,
I know this is an obsolete technology, but I came across some data produced on the 454 instrument (archived in SRA/ENA) which I'd like to use...
163 results in 0.0037 seconds.
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454 GS FLX Titanium 'paired end' protocol
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displaying 454 data on ucsc
How can i display the 454.bam file on the ucsc custom tracks?
I have the contigs.bam file from the 454mapper software. Thare is no index file... -
how to remove first 15 bases of a sequence using unix or qiime
So I'm pretty sure all of my sequences have a 15 base pair adaptor sequence at the beginning, and I'd like to remove it, so then my downstream analysis... -
454 and bacterial genome assembly Q
Hello - Forgive me if this question has been asked many times.
I have 454 bacterial genome sequence data from clinical isolates from which... -
Edge-pro error
Hi all,
I recently downloaded the software EDGE_pro_v1.3.1 and when trying to run it:
OMP_NUM_THREADS=6
edge.pl... -
Quantification Alternative
Hi guys,
I have recently been performing runs on the Roche GS Junior platform. I am using the platform for viral discovery from affected... -
Denoising of 454 Data Set - Flow pattern B
I am working with a data set that uses flow pattern b and hence, Qiime and AmpliconNoise cannot be used for the denoising step:
"Since late... -
Demultiplexing 454
Dear all,
I am trying to de-multiplex a 454 fasta file.
It is a public data file. In the paper (PMID:21731642) the authors say... -
Running a 454 library in Ion torrent?
Hi all,
We have a shotgun library prepared for 454 and would like to run it in ion torrent for extra data. We learned that the sequencing... -
statistical analysis of 454 bisulfite sequence data - small sample size
Hello everyone,
I am trying to figure out how to analyse some bisulfite sequencing data that I have and I am hoping that someone will have... -
454 ACE assembly with paired reads... how to view?
Hi,
I'm new here, thanks in advance for any help.
I have a 454 dataset of 'allcontigs.ace'. The reads consist of paired and... -
454
I would like to sequence 16s from more than 400 sample of nasopharyngeal samples by pyrosequencing.
It seems to be a good method to do... -
All Data Disappears After Run
We had our 454 upgraded several weeks ago. Now when the run completes something deletes all of the data files from the instrument. The log files show... -
fastq files, lowercase and base score
I am working with a bunch of fastq files from various sources. I do not know anything about the sequencing type. I have found that some have not been... -
Extract sequence from single OTU 454
Hi everyone,
I would like to extract a single sequence corresponding to an OTU obtained in the analysis of the 454 pyrosequence.
...
