Tweet
All Data Disappears After Run
We had our 454 upgraded several weeks ago. Now when the run completes something deletes all of the data files from the instrument. The log files show the run was completed and the data and image files were written to the disk, but they are just not there. This has happened twice since the upgrade. Has anyone else had their data disappear like this or have any idea what could be happening?
-
-
You or your technician did not install/edit the backup script which is ran at the end of sequencing. It is usually used to copy data from the sequencing machine (it's computer) onto a server machine. Look into /data directory on the sequencer machine. -
Well, this is usually the 'postAnalysisScript.sh'. This should be edited to match your needs/environment. As martin2 has already mentioned, have a look at /data on your machine. AFAIK the system (default) is not configured to automatically remove runs from the systems when they have been (successfully) copied to the analysis server.Comment
-
The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i
...01-24-2023, 01:19 PM -
Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.
The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes...01-09-2023, 03:10 PM
Comment