Hello,
Can you please help me understand what is the function of NEBNext adapters (hairpinloop adapters) during library preparation. Please...
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PCR after size selection of cDNA library
Hi all,
Recently I'm trying to make cDNA library using Tag-seq protocol.
After amplifying my sample with illumina adapter, I select...
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MiSeq still underclustered at 18 pM
Hello dear people,
We are doing some whole genome sequencing on a plant genome using the MiSeq with v3 reagents for 2x250 paired-end. Library size...
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sequences proceeding Illumina adapter
My question: If I have a a 24 nucleotide long sequence before the sequencing adapter would my DNA fragment still attach to the flow cells? Is there a...