Hello
I am new in RNA-seq analysis and it is difficult for me to find out info about quality assessment in the matter. I did an analysis...
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Illumina paired end adapter contamination problem
Hello everyone!
I have rna-seq Illumina paired end reads and want to proceed with adapter trimming.
I have some confusions:
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adapter contamination in reads?????
hello everyone,
I would like to ask one qus: why am I getting 3'adapter sequences in R1 and R2 reads (as according to seq...
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Adapter/primer trimming from RNAseq reads
Our lab had received RNAseq data from Illumina. I was able to do preliminary quality analysis using Fastqc app. Results are back however there seems to...
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Data Clean & Adapter Contamination
Hello All,
I am currently starting to analyse my RAD data set (Illumina HiSeq, 150bp PE sequencing run). I have used Trimmomatic for the...
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FastQC; overrepresented sequences versus a grep
Hi,
I have an odd observation with fastQC's figure for over-represented sequences versus the number I get out when I do a simple egrep...