Hello,
I have been using the Kapa Hyper Prep kit to try and prep DNA libraries for target enrichment.
I am using custom Y adapters and...
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No amplification with Kapa kit
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FASTq from Torrent Server
Do Fastq files exported from IonTorrent Server have adapters and barcodes already removed? Should I use cudapt or trimmomatic to get rid of them? -
Extract reads from paired-end fastq based on specific adapters with bbduk
Hello everyone, I am using bbduk.sh (from bbmap toolkit) to extract reads from paired-end fastq files based on the presence of specific adapters in the... -
QIAseq FX DNA Library Kit adapters for trimming
Can anyone point me to the sequences of QIAseq FX DNA Library Kit for adapters trimming in preprocessing? Illumina has exact sequences for trimming included... -
trimming BGI adapters
Hello,
I have paired-end 100bp reads generated from BGI-seq 500. The sequencing center did some adapter removal trimming before delivering... -
NExtflex adapters for Truseq
I am preparing RNA libraries using the TruSeq Total RNA kit from Illumina and I am trying to use NextFlex adapters in lieu of the truseq ones. I am stuck... -
Trimmomatic: Not trimming..
Hey! I am trying to use Trimmomatic to trim Illumina adapters sequences from my TruSeq Small RNA library SE 75bp reads off of a HiSeq but I am unsuccessful......Last edited by lchippy; 11-12-2017, 11:34 AM. -
Role/Importance of adapter contamination in cDNA libraries
I have created cDNA libraries from Drosophila melanogaster mRNA using the KAPA mRNA HyperPrep kit. The size distribution on the Bioanalyzer looks good,...Last edited by patkrat; 08-12-2017, 08:03 AM. -
Questions about RADseq oligo
I will be genotpying ~500 samples using the original RADseq protocol, and I have a couple of questions.
First, is desalting a sufficient... -
Why A and B primers in SAGE? Why not just one?
Reading about SAGE (serial analysis of gene expression). Everywhere discusses adapters having A or B primer but no one explains explicitly why dividing... -
adapters
how to get universal adapters for illumina 1.5 for the cutting the adapters site of fastq file -
Remove Illumina TruSeq Index adapters
Removed because cross-posted to Biostars.Last edited by enrico16; 01-14-2015, 02:19 AM. -
Do not know Adapters inside Sequences
I have ~100 Bissulfite-Seq fastq files and ~100 RNA-Seq fastq files representing many samples. All of the seq files were produced by Illumina tech. ... -
Sequencing PCR amplicons on MiSeq
Hi,
I'd love to receive some advice on a strategy to sequence PCR amplicons on a MiSeq.
I'd like to simultaneously sequence... -
Running a 454 library in Ion torrent?
Hi all,
We have a shotgun library prepared for 454 and would like to run it in ion torrent for extra data. We learned that the sequencing...
