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Jeflo started a topic Problems with adaptor ligation - NEBNExtUltra II

in Sample Prep / Library Generation

07-01-2021, 04:52 AM
Problems with adaptor ligation - NEBNExtUltra II

Hello to you all,

I want to perform DNA seq and I am having issues in the step of ligating the adaptors to the sequences.

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GMDickson started a topic Preparation for amplicon sequencing without PCR

in Illumina (Solexa)

07-26-2017, 09:19 AM
Preparation for amplicon sequencing without PCR

Hi all,
I have had a good look through the forum posts and don't seem to find the same questions I have about preparing amplicons for sequencing...

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sunnymercy started a topic Is end repair and 5’phosphate necessary for the adaptor ligation?

in Illumina (Solexa)

08-06-2015, 12:56 AM
Is end repair and 5’phosphate necessary for the adaptor ligation?

Hi,everyone! I’m new here and this forum has helped me a lot,thanks.
We are now hoping to carry out NGS of PCR amplified DNA fragment. The DNA...

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