Hello all,
I have had several troubleshooting attempts at sequencing a low-diversity, small amplicon library on a NextSeq2000 using P1 50...
3 results in 0.0033 seconds.
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Small amplicon library sequencing
Last edited by CitizenSnips; 09-08-2025, 06:16 AM. -
How to handle Ns in the middle of reads
For my illumina data fastqc shows presence of N's at positions 13,14,15 in 101 bp longs reads. If i go for cropping first 15 bases by using trimmomatic,...Last edited by themysticgeek; 01-08-2014, 02:55 AM. -
Ambiguous bases vs mismatches in Tophat
Does Tophat treat ambiguous bases (N) in the reference as mismatches? In the Tophat manual -N/--read-mismatches is defaulted to 2 but there doesn't seem...
