33 results in 0.0027 seconds.
-
MiSeq i100 vs MiSeq (OG)
Soon, we may have to replace our MiSeq (OG), if the EOL notice is too be believed. For those who have switched to the i100, or i100+, what are your thoughts... -
16S V4 reads contain high % reverse primer
Hi everyone,
I am running BI analysis for 16S V4 samples that was run using 515F/806R, MiSeq 300 PE. In average, all samples have more...Last edited by Afina M; 05-18-2021, 07:15 PM. -
Too little PhiX - problem with denaturation?
Hi all,
first the TLDR:
Do you know of any factors that might cause PhiX denaturation to fail or reverse, outside of typical mistakes... -
Minion sequencing
Hi all,
I have a very basic question. We have ±10 samples that we would like to sequence using MinION. These are PCR amplicons that we... -
metagenomics tool
Gaia 2.0 is now online:
- valid for any type of analysis (16S/18S, ITS, WGS, metatranscriptomics)
- valid for any type of sample... -
Sequencing the unknown region of a large amplicon
We inserted a tag into the genome of human cells and made clonal cell lines. We can tell by PCR that most of the clones have an insert that is much larger... -
NexteraXT kit on cDNA amplicon?
Can I use the Nextera XT library prep kit on a 2kb cDNA amplicon? Just wanting to check that the tagmentation process will work ok on cDNA. I've only... -
Selections of library preparation based on panel for MiniSeq
Hello everyone,
I want to make a targeted sequencing experiment for screening mutations which are involved to colorectal cancer. I think that using... -
Amplicon Sequencing & Reads Per Sample (Coverage)
Hello,
Is there a "best" way to normalise and pool amplicon samples to acquired even, or balanced read coverage. Otherwise... -
amplicon runs using bcl2fastq v217
Has anyone noticed issues with quality scores on amplicon runs since the new version of bcl2fastq came out? I've had a number of runs be questionable... -
SequalPrep normalization plates and low amount of starting material
Hi,
I have been looking into Invitrogen's (ThermoFisher Scientific) SequalPrep normalization plates for a quick way to clean and normalize... -
Amplicon resolving differently between bioanalyzer and tapestation
Hello,
We are seeing a discrepancy between the Bioanalyzer and Tapestation for a large amplicon product that we received for sequencing. We received...Last edited by Pab77; 01-25-2016, 12:13 PM. -
Truseq Custom Amplicon kit overhang sequences
I have been searching for for the overhang sequence on the i7 and i5 indices of the Truseq Custom amplicon Index kit, but couldn't find them..
... -
Squeezing the most out of Nextera XT...?
Hi All,
I'm wondering if anyone has experimented with pushing the limits of Nextera XT in order to get more samples out of a kit? I want... -
PacBio Amplicon reads assembly
I've got a question regarding the assembly of PacBio reads. We've created a library of approximately 5000 different amplicons between 1 and 3kb and successfully...
