Here is something that puzzles me:
I have 4000 reads that align to my reference genome. I can visualize them, they cover maybe 80 % or a little...
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reads align but contigs do not??
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Cufflinks did not assembly a mark-gene ! Any solution?
Hi,
I have Hi-seq directional paired-end data. I used the standard pipeline based on their "Nature protocol" paper to assembly...
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How to tell what is the 'best' assembly
I'm assembling some bacterial genomes for which there are no close reference sequences available. Reads are 70-90 bp after cleaning and trimming with...
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Assessing quality and accuracy of de novo genome assembly
All,
I am curious whether anyone out there has a method for assessing the quality and accuracy of de novo genome assemblies? I am currently doing...