De novo discovery

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infering isoforms in merged transcriptome by Owens Started by Owens, 10-11-2022, 07:30 AM	1 response 49 views 0 likes	Last Post by Lariviere 10-11-2022, 09:55 AM
Resources to learn genome assembly workflow for small genomes (like viruses) by Rezaeir Started by Rezaeir, 08-16-2020, 08:43 AM	0 responses 21,428 views 0 likes	Last Post by Rezaeir 08-16-2020, 08:43 AM
Regeneration of Pair End MiSeq flowcell by Saurabh_Raj Started by Saurabh_Raj, 06-04-2020, 03:56 AM	2 responses 5,913 views 0 likes	Last Post by Guest 06-05-2020, 01:19 AM
Contig and scaffold N50 by jm1789 Started by jm1789, 11-12-2019, 01:33 PM	2 responses 8,222 views 0 likes	Last Post by Guest 11-13-2019, 06:19 AM
de novo assembly using Velvet to reconstruct a PCR amplicon by guineverelee Started by guineverelee, 08-13-2019, 03:27 PM	7 responses 10,816 views 0 likes	Last Post by guineverelee 08-16-2019, 11:25 AM
SPAdes duplicate contigs between chromosome and plasmid by causland Started by causland, 08-09-2019, 07:58 AM	0 responses 5,879 views 0 likes	Last Post by causland 08-09-2019, 07:58 AM
genome assembly polisher that can use Sanger reads? by ssully Started by ssully, 03-20-2019, 10:25 AM	1 response 6,581 views 0 likes	Last Post by JTH-Genome 06-27-2019, 09:55 AM
FastQC interpretation by ifyouSEQelaine Started by ifyouSEQelaine, 04-25-2019, 07:14 AM	1 response 6,470 views 0 likes	Last Post by GenoMax 04-25-2019, 07:41 AM
Denovo identification of Centromere and Telomere by Jassipox Started by Jassipox, 06-17-2017, 06:35 AM	2 responses 10,662 views 0 likes	Last Post by JTH-Genome 04-09-2019, 06:09 AM
long-range technologies for genome scaffolding by ybukhman Started by ybukhman, 02-09-2018, 01:12 PM	3 responses 8,733 views 0 likes	Last Post by JTH-Genome 04-09-2019, 05:59 AM

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ESPRESSO: Quantifying transcript isoforms from long-read sequencing by seqadmin Started by seqadmin, 01-23-2023, 12:48 PM	0 responses 28 views 0 likes	Last Post by seqadmin 01-23-2023, 12:48 PM
Top 11 Takeaways from JP Morgan Healthcare 2023 by seqadmin Started by seqadmin, 01-18-2023, 04:23 PM	1 response 115 views 0 likes	Last Post by OlgaCaswell 01-25-2023, 05:27 AM
Long-Read Sequencing: Nature’s Method of the Year 2022 by seqadmin Started by seqadmin, 01-13-2023, 03:34 PM	0 responses 33 views 0 likes	Last Post by seqadmin 01-13-2023, 03:34 PM

A Brief Overview and Common Challenges in Single-cell Sequencing Analysis

by seqadmin

The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations¹, cell lineages², cellular diversity and regulation³, and development in multicellular organisms⁴. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i...
- Channel: Articles
01-24-2023, 01:19 PM
Introduction to Single-Cell Sequencing

by seqadmin

Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.

The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes...
- Channel: Articles
01-09-2023, 03:10 PM

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