Hello Everyone,
Can anyone provide me with some references to the methodology for isolation of only bacterial RNA from the host cells (in...
11 results in 0.0027 seconds.
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Total RNA-seq of intracellular gram-negative bacteria
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Bacterial scRNA-seq experience?
Hi all,
I'm trying to perform a scRNA-like library prep in bacteria, so I cannot take advantage of the polyT reverse transcription primers.... -
SE vs. PE for bacterial RNAseq
Hello,
We plan an RNAseq analysis to study gene expression in a bacteria that we have an in house reference genome assembly (~7.8Mbp) and gene... -
Why is rpfm of bacterial genomes much higher than eukaryotes
I found the rpkm of my RNA-seq datasets of my bacterial samples much higher than eukaryotes. The rpkm for bacteria is usually 50 or even more than 100.... -
Bacterial RNA-Seq Differential Gene Expression Biological Reps vs Exp Conditions
We have 6 bacterial samples that we are working with - 2 sets of 3 biological replicates (condition A -1, 2, 3 and condition B 1, 2, 3)- that we performed... -
Negative RPKM values by EDGE-pro
Hello,
I am using your EDGE-pro programs for RNA-seq analysis
of bacterial species.
I could run the program, but I got negative... -
Is this edgeR code OK?
Hi,
I would you to revise my edgeR code since it's possible I missed something important because being quite new on this.
... -
Differential gene expression (RNA Seq analysis MiSeq paired end reads 250 bp)
Hi All,
I am relatively new to bioinformatics, therefore your expertise could help me how to go about RNA Seq analysis.
I have two... -
Lots of chimeric transcripts Ion PGM®
Hello Bioinformatic community,
We have recently sequenced a bacterial transcriptome with 316 chip from IonTorrent (1.5 million sequences).... -
Create GTF or GFF3 file from draft genome
Hello Bioinformatic community,
I recently entered in RNA-Seq world, I’m a second year PhD.
We have recently sequenced a bacterial transcriptome.... -
Optimal fragmentation of bacterial mRNA
Would any one please share conditions used for fragmenting bacterial mRNA? I am shooting for ~200 nt and starting mRNA conc. is ~75-100 ng/. I am using...
