Hello,
We received the raw reads of our ddRAD library from a sequencing facility, and after demultiplexing, I noticed that there is a 10-fold...
28 results in 0.0030 seconds.
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ddRAD raw read count difference by barcode
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Demultiplexing IONA® generated data
I'm working on my MSc thesis (bioinformatic analysis of NIPT data) and I don't have access to reliable NIPT data, but recently I achieved some multiplexed... -
Split seq data based on index AND barcode
Dear all,
I am currently designing an experiment, where my samples will have both a barcode and an index.
Barcode: Sample-specific...Last edited by Meyana; 01-26-2020, 08:25 PM. -
Advice needed for --use-bases-mask option (bcl2fastq)
Hi all,
A colleague in the lab asked me to demultiplex a recent NextSeq run. She loaded it with samples prepared from two libraries. One... -
Sample sequencing performence depending on index?
Hi,
I have some Illumina exome data from different runs on NextSeq. I see quite some difference in the number of produced reads per sample, which... -
Good index sequences for unique dual indexing?
After the index-hopping scandal (see Illumina's white paper) it sounds like the field is moving in the direction of unique dual indexing, i.e. dual indexing... -
Index (Barcode) Lengths - Are they ALL Even?
Are all index/barcodes of EVEN length? For example, 6 bp, 8 bp, 10 bp, 12 bp?
Has anyone ever come across ODD index lengths? 5 bp, 7 bp?... -
Custom multiplex sequencing barcodes / indexes (8-mer)
Hi all,
I have synthesised a custom set of Illumina adaptors that use an 8-mer index to allow 384-plex multiplexing. I got these index... -
Multiple files with same barcode in TCGA
Does anyone know what differentiates the bam files from samples with the same barcode? in TCGA For example, this sample (TCGA-D7-8575-01A-11D-2340-08)... -
inline barcodes appearing in reverse read of pair
Hi All,
I have Illumina 101bp paired-end data where the libraries were prepared with custom inline barcodes at the P5 end. So the first... -
Barcode Contamination - iChIP
Hi everyone,
I have attempted to perform indexing-first ChIP (iChIP) as described here: http://www.ncbi.nlm.nih.gov/pubmed/25103404. The... -
Make your own libraries/barcodes?
Hello,
I am new to sequencing, and plan to perform genome-wide CRISPR/Cas9 screening as described in this paper - http://www.ncbi.nlm.... -
Splitting fastq file by barcodes without producing unmatched.fq file?
G'day Everyone,
I am trying to split six plates of data by the barcodes of 576 indviduals. We are split our data using 'fastx_barcode_splitter.pl',... -
Illumina_PE_reads-how to add the barcode sequence of read1 to the beginning of read2
I have read1.fastq and read2.fastq from the same lane.
At the beginning of read1, there are 7-9bp barcodes, but no barcode in read2.
so... -
Do illumina fastq file have barcode and adaptor?
Hi all,
I saw this thread http://seqanswers.com/forums/showthread.php?t=8176. It said that in illumina fastq file, there is no barcode. But when...
