7 results in 0.0030 seconds.
-
Re-basecalling Illumina data to improve accuracy?
With Oxford Nanopore data, basecalling is a crucial and well-documented step, using one of their assorted free and open-source basecaller algorithms for... -
Wierd base calling on MiniSeq run
We recently ran some libraries on the MiniSeq (2 x 150 bp Mid Output kit) and got some weird traces for the %base graph (see image attached). The other... -
mpileup sample specific read count/quality info
Hello,
I am looking to use mpileup for base calling. Previously I ran samples together but could not find any sample specific read count/quality... -
Base call pairs drifting apart over the course of a MiSeq run
Hello everyone,
We've been having problems with our base calls, especially A and T, diverging over the course of a run (see attached screen... -
High Error Rate After Repeat In Reads
I have obtained whole genome sequencing data for human patients. I notice that after a repeat region, the rest of the read is typically soft-clipped.... -
fastq quality values
Hi All,
We have a couple of questions related to the fastq format and quality values:
1. We have been looking at the quality values... -
Would you use the SolexaPipeline or an external base caller?
Hello,
A few of my lab co-workers and myself are interested on working with some GAIIx data from a bacterial genome. And well, we want...
