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  • hjarnek
    Junior Member
    • Jan 2024
    • 3

    Re-basecalling Illumina data to improve accuracy?

    With Oxford Nanopore data, basecalling is a crucial and well-documented step, using one of their assorted free and open-source basecaller algorithms for different levels of accuracy and speed. But for Illumina data, basecalling algorithms are barely ever mentioned. How come?

    You used to be able to get .CIF files (Cluster Intensity Files), containing the raw signal values of the different dyes, and being the main input of any basecaller program. But these don't seem to be supported anymore, at least not for high-throughput technologies like HiSeq/NovaSeq. I wonder, is it still possible to do your own basecalling on Illumina data, and which alternative algorithms exist that actually offer an improvement of accuracy?

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  • seqadmin
    Pathogen Surveillance with Advanced Genomic Tools
    by seqadmin




    The COVID-19 pandemic highlighted the need for proactive pathogen surveillance systems. As ongoing threats like avian influenza and newly emerging infections continue to pose risks, researchers are working to improve how quickly and accurately pathogens can be identified and tracked. In a recent SEQanswers webinar, two experts discussed how next-generation sequencing (NGS) and machine learning are shaping efforts to monitor viral variation and trace the origins of infectious...
    03-24-2025, 11:48 AM
  • seqadmin
    New Genomics Tools and Methods Shared at AGBT 2025
    by seqadmin


    This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.

    The Headliner
    The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...
    03-03-2025, 01:39 PM

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