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Help needed to prepare consensus including overhangs
I am having trouble merging my pre-trimmed fastq files to merge with the inclusion of the overhangs/nonoverlapping reads at either end. have many files...
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Calling BBMerge from Java program
Hi,
I have a handful of metatranscriptome sequences that I would like to merge using BBMerge, and I've sorted them together into bidimensional...
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bbmerge detects different adapters
Hello,
I have some bacterial genomes which were sequenced using the Nextera Flex library prep kit ( insert size ~350 bp) and MiSeq paired...
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Introducing Clumpify: Create 30% Smaller, Faster Gzipped Fastq Files
I'd like to introduce a new member of the BBMap package, Clumpify. This is a bit different from other tools in that it does not actually change your...Last edited by Brian Bushnell; 12-04-2016, 12:23 AM.
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bbmerge mismatched
Hi,
While merging 16S pair-ended miseq data (2x250) i would like to disable ambiguous bases. BBmerge documentation states the following "When...
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bbmerge ratiomode ?
Hi,
bbmerge seems to use by default the ratiomode as follows:
ratiomode=t Newer algorithm. Slower, but higher merge rate....
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Introducing Tadpole: an assembler, error-corrector, and read-extender
Tadpole, a new BBTool, is an extremely fast kmer-based assembler. How fast is it? Around 250x faster than SPAdes with --careful (which is how we generally...Last edited by Brian Bushnell; 10-14-2015, 05:49 PM.
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Comparing Merged Seqs to a Reference Seq
Hello,
I recently received paired-end reads from an amplicon library I sequenced with Illumina. I used BBMerge to merge the paired-ends...
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Introducing BBMerge: A paired-end read merger
I'd like to introduce a new read-pairing tool, BBMerge, and present a comparison with some similar tools, FLASH, PEAR, COPE, and fastq-join. BBMerge...