Hi guys,
I have two queries about the homemade AMPure XP beads.
1) Almost in all the homemade recipe of AMPure XP beads, there...
14 results in 0.0022 seconds.
-
Homemade AMPure XP has some problems
-
Streptavidin Bead Quantitation
Hi Everyone!
I am trying to evaluate how well our new robot handles bead clean-ups with streptavidin beads. I would like to objectively quantitate... -
Using Clean NGS from proteigene for RNA clean up of single cell
Hello,
Does anyone have some experience using Clean NGS beads from proteigene instead of RNA clean XP beads from Beckman for single cell application?... -
Alternatives to Pippin Prep for ddRAD?
My lab is interested in doing population connectivity/phylogeography on herps, and think that ddRAD is probably the best NGS option for us (since we don't... -
Question about library normalization methods
Hi all,
I am looking for a bead based library normalization method as an alternative to the library normalization in the NexteraXT kit.... -
jennifer_jy started a topic What is the material and size of micro-bead used in ION Torrent 314-318 chipin Ion TorrentWhat is the material and size of micro-bead used in ION Torrent 314-318 chip
Our Lab decided to purchase micro-bead for ION Torrent 314,316,318 chip, but it's hard to find the detail information of the size and material of the... -
Weird Ampure XP problem - gelling
We tried to purify some PCR-products in preparation for library construction for Illumina sequencing. For several samples, after mixing the beads and... -
BEADS vs PYICOS
Hi,
I have ChIP-seq SOLiD sequencing data with a relatively low amount of reads and I would like to extend the length of the fragments.... -
Ampure bead clean up
Hello,
How much drying time do you normally allow when using ampure beads XP to clean up your library preps?
I've been using the... -
Nextera XT Normalisation
Hi all,
I have been using the Nextera XT kit to do my library preps for MiSeq sequencing of fairly low complexity libraries and have been... -
Removing Adapter Dimers with AMPure Beads
Can someone help?
I have adapter dimmers or some other contaminant below 200 (probably 170bp). Ultimately, my goal is to remove the contaminates... -
Agencourt RNAdvance Blood kit
Hi, I'm using a blood RNA kit from Agencourt that uses 2 binding buffers, the first to bind to the magnetic beads and a second to rebind after the DNase... -
PCR duplicates increase when excess of beads
Hello,
We are experiencing an increasing of PCR duplicates.
Could this be realated to an increasing of the quantity of beads in order to... -
low yield of SOLiD templated beads
Hi
We have been facing problems with solid 3 plus to come up with sufficient beads for deposition. We hope to sort this out before moving to solid...
