Hi all
I m about to sequence 50 samples (shotgun metagenomics) from animal feces (complex metagenomes) using the novaseq 6000: apx 2.5billion...
19 results in 0.0029 seconds.
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If I use different type of samples in a seq lane - will that introduce a bias?
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Poly(TA) reads in NGS libraries
Hey all,
Recently, our lab is generating RNA-Seq and ChIP-Seq libraries that contain very high amounts of reads containing a poly(AT) sequence.... -
Bias due to DNA extraction kit and illumine version used
Do you think DNA extraction kit and illumine version used could be a source of variation?
Our idea is to perform genome comparison with bacterial... -
Non-random Illumina sequencing error?
We have observed a couple of relatively frequent (1:4) SNPs in what is supposed to be a clonal culture. The simplest explanation is that it's not a clonal... -
HiSeq 2000 Size Bias
We have recently switched to sequencing paired end 250, and noticed that there is always a bias towards the smaller fragments. We made sure that our... -
DESeq2: Need advice modelling a multi-factor analysis
I would like to delete this post. -
increased bias in single-buffer library preps (NEB, KAPA,...) Reason for concern?
Hi All,
the latest generation of library prep kits seem very convenient (only a single cleanup) and promise higher complexity sequencing... -
[FASTQC] Biases in GC whole sequence content
Hey all, I am analyzing several RNA-Seq datasets and have noticed somewhat of an odd pattern. In all of the samples I've QC'd so far, there is a bias... -
How to subtract a coverage track of mock IP from a set of ChIP tracks?
I have a set of ChIP data, most of them with a transcription factor, but also control data, which was not the raw input chromatin, but instead they performed... -
Biases in NGS Library Prep
Clearly there are biases in NGS library prep: http://blog.genohub.com/consider-bia...y-preparation/
Has anyone experienced... -
Position-specific GC bias?
Hi, I've been having an unusual problem with my reads. I've been doing exome sequencing on a HiSeq 2000, but I'm getting only about 40% GC content at... -
non tagged strain biases with HA antibodies
Hello,
I'm currently encountering a problem with my ChIP-seq analysis, for some highly expressed genes I have a peak on the ORF in the non tagged... -
Barcodes & biases
Hi there,
Has anyone observed barcode-specific biases in his/her NGS samples?
I am aware of the miRNA-seq papers on that... -
barcodes from recent papers...better?
Hello,
I've been wondering what barcodes to use for some 454 amplicon sequencing: A couple of new publications confidently suggest that their... -
RNA-seq reads counting: sum or max
Hi guys,
From first time I entered RNA-seq analysis, I always count the total number of reads that map to certain genomic area (with coverageBed...
