Dear all,
I have recently sequenced RNA populations from two different cell types that were separated using FACS (negatives and positives). The...
18 results in 0.0029 seconds.
-
Weird biological replicates
-
how does cuffdiff calculate replicate FPKM values?
Hi,
I have two conditions, 5 replicates for each. After running cuffdiff I noticed the FPKM values in gene_exp.diff for one condition do... -
Bacterial RNA-Seq Differential Gene Expression Biological Reps vs Exp Conditions
We have 6 bacterial samples that we are working with - 2 sets of 3 biological replicates (condition A -1, 2, 3 and condition B 1, 2, 3)- that we performed... -
Sequence in (biological) duplicate or not?
Dear all,
I am wondering: how much (methodological) advantage would it have to RNAseq in duplicate (i.e. 2 biological replicates) compared... -
RNASeq Biological Replicates
Hi all,
I have RNASeq data for 20 different samples (human cell types and tissues). 10 of these samples are represented by single biological... -
Replicates correlation for differential expression
I have miRNA seq experiment with two conditions; (i) Asthma patients (treatments) and (ii) normal (controls). There are 9 human patients and 6 normal...Last edited by seq_finder; 11-22-2013, 07:44 AM. -
Q values in cuffdiff with 2 biological replicates
I'm using cuffdiff 2.1.1 to look for differential gene expression between two conditions. Each condition has 2 biological replicates. The results I get...Last edited by nr23; 08-22-2013, 10:30 AM. -
MACS2 ChIP-SEQ ANALYSIS WITH BIOLOGICAL REPLICATES
I am having problems with the broad mark H3K27me3, my biological replicates and identifying differential enrichment between treatment groups
... -
Cuffdiff with biological replicates problem
I am trying to perform the cufflinks/cuffdiff workflow on the Galaxy server. I am comparing expression at 2 times (Day 2 and Day 6) and 2 conditions... -
When to merge specify replicates
Hi,
I've got RNA-Seq data (pe 100bp) from several biological replicates and 11 different conditions and want to compare DE between conditions....Last edited by nr23; 06-05-2013, 01:49 AM. -
Using DESeq with Biological Replicates with Different RNAseq Read Lengths
Is it okay to use DESeq with count data from biological replicates having different RNAseq read lengths?
thanks -
How to work with biological replicates
Hello everyone,
I am working with small-RNA(~50ntds) cancer samples, RNA-seq data. I have tumors and normal cell samples with biological... -
biological replicate variation assessment
Hi to everyone,
I have rna seq data (bacterial) grown in rich and poor growth mediums. we have carried out 2 replicates of each of these conditions.... -
Biological replicates with very different number of reads
Hi,
I want to do a differential analysis between two conditions and I have 3 biological replicates per condition. All samples have similar number... -
Which Noramlization would be best?
Hi friends,
I have RNA-seq data for 5 controls and 5 cases (biological replicates). I want to normalize (between sample normalization)...
