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Aligner for PacBio RNASeq
I am wondering if I can use BLASR for aligning RNASeq reads from Human cell lines. I am interested in identifying the splice sites. I read this paper...
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problem with samstat on PacBio alignment
Dear all,
I ran an alignment of Sequel data using blasr. Now I want to get some metrics about the sam file using samstat 1.5.1 (running on Ubuntu...
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Tool to find reads bridging two scaffolds?
I have a set of PacBio reads and I need to find which of them actually bridge two scaffolds of an existing assembly.
So far I've tried blasting...
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HGAP error caused by blasr memory limit
Hi,
I am using HGAP for a large genome pacbio data.
I do not have cluster and our server has 64 cores & 500G mem.
In the smrt...
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Get consensus sequence from blasr alignment
Hello,
I have some pacbio reads which I have aligned to a reference sequence using blasr. I can see the resulting alignment in igv with...
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BLASR - Alignment to reference without gaps
Hi there,
is it possible to align reads to a reference genome with BLASR using gaps only in the reads, but not in the reference genome?...