Hello,
I'm sorry if this is a naive question, but can somenoe explain to me the practial difference between performing a blast using the API...
37 results in 0.0032 seconds.
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Differences between api call to blast and local blast
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Return not aligned with BLAST
Hello,
I am new to Seqanswers, though not to Bioinformatics.
I have a doubt with BLAST. Is there a simple way to get all... -
Creating blast database blast+
I want to create a database consisting of all many nucleotide sequence fasta files in blast+ linux tool ,but the tool only allows me to create database... -
Use of cutomized blastn datagase
I have installed ncbi-blast-2.3.0 in my PC
I have created a custom database (I think it is made of the 6 new files named after my database name... -
Problems with BLAST+ HSP
Dear Community
I have a very annoying problem
. In our laboratory we’re going to launch automated BLAST-searches. Therefore we’re...
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blastn help for primer alignment to custom db
Hello!
I am trying to find primer pairs that will amplify across 6 individuals of the same taxa. I have forward and reverse primers(<30bp... -
How to convert blastn output file to sam/bam
I am trying to convert the output of blast in a .sam or .bam file using the blast2sam tool.
The alignement of the reads has been done with... -
Download BOLD sequences with R and remove duplicates
Hello,
I am using the package bold in R to download COI sequences from BOLD and specifying the taxon of interest and the marker like this:... -
Troubleshooting installation of BLAST+
Hi!
I've got a copy of ncbi-blast-2.2.30+.dmg. The installer runs as expected and reports successful installation to my HD. However, I... -
How can I write a bash command to blast local nt database?
I have been reading this.
I was able to write a basic query using blast n as follows:
...Code:blastn -db ~/programming/blastexample/db/tdb
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Standalone BLAST results
Hi,
I used to standalone BLAST to subtract the Clostridium difficile proteome from the human proteome, using the command
>blastp... -
Running Blast+ on multiple nodes on a cluster -- what is the best way to that?
Hi, I have been recently granted access to the HPC cluster of my university. I am going to run several blastx searches (Blast+ version, not legacy blast)... -
Installation of BLAST+ without root privileges
Hi,
I was trying to install BLAST+ as a dependency to an error correction tool SPRAI into a cluster server which I do not have root privileges,... -
Blast to reference genome
Hello!
I have a bit of a conundrum with Blast of all things. I have it locally installed on a Mac. I have individual fasta files of sequences... -
tblastx in blast+ gives an error regareding a DB alias but blastall works fine
I'm trying to run `tblastx` locally. For this I created my own database using the command:
`makeblastdb -in all_seqs.fasta -dbtype nucl -title...
