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anna_m started a topic Blue pippin size selection elution volume

in Sample Prep / Library Generation

07-03-2015, 02:20 AM
Blue pippin size selection elution volume

Hello,
Has anyone has tried eluting their TruSeq DNA libraries in a smaller volume than the 40uL stated in the protocol?

We carry...

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avo started a topic TruSeq PCR-free transition

in Sample Prep / Library Generation

11-15-2013, 01:57 AM
TruSeq PCR-free transition

Hi together,

my lab is planning to switch to the TruSeq PCR free library prep. We would like to perform size selection on a bluepippin. Reading...

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Last edited by avo; 11-15-2013, 04:13 AM.
anna_m started a topic Bimodal distribution in TruSeq libraries

in Sample Prep / Library Generation

10-08-2013, 05:11 AM
Bimodal distribution in TruSeq libraries

Hi,
I made some control TruSeq genomic gel-free libraries using Ecoli and pooled these together equimolar. This pool then underwent blue pippin...

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anna_m started a topic Size selection for TruSeq DNA libraries

in Sample Prep / Library Generation

06-17-2013, 05:54 AM
Size selection for TruSeq DNA libraries

Hello,
When is the best time for carrying out size selection of TruSeq DNA libraries - pre or post PCR? What kind of adapters are labs generally...

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