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Kaizen started a topic Recommended read depth for low input bulk rna-seq

in Bioinformatics

02-27-2019, 12:33 AM
Recommended read depth for low input bulk rna-seq

Hi,
I'm wandering what is the sequencing depth recommended for low inupt bulk RNAseq, let's say 1000 cells.
I found on the Immgen website...

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chris11 started a topic nextera xt library prep for bulk RNA-Seq of mouse

in Sample Prep / Library Generation

04-21-2016, 08:59 AM
nextera xt library prep for bulk RNA-Seq of mouse

Hi All,

We purchased an illumina nextera xt DNA library prep kit and accompanying 96 index kit with the goal of doing single cell RNA-seq...

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Last edited by chris11; 04-21-2016, 09:01 AM. Reason: typo
stvos started a topic Genome assembly of a DNA bulk of 100 individuals

in Bioinformatics

07-29-2013, 04:21 AM
Genome assembly of a DNA bulk of 100 individuals

Hi,

does anyone have experience with assembly of bulks?
It is not an ideal database to start from, but would anyone know some methods...

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