10 results in 0.0030 seconds.
-
Cot DNA in capture
We normally add 5 ul of Cot DNA in our target capture. I wonder if anyone has experience forgetting to add Cot DNA in capture, or add less amount (40%)... -
Using Restriction Enzymes pre library prep for targeted sequencing.
Hey Everyone,
I'm using a targeted capture approach for sequencing the variable regions in Ig, we're trying to develop a basis for genotyping... -
EZ SeqCap
Can you hybridize longer than the 64-72 hours that Nimblegen-Roche recommend? Anyone have any problems with that long of a time? -
Compare difference capture technologies
Please let me know if I'm posting in the wrong place.
My question that since there are so many different exome capture technologies,... -
Low exomic yeld after capture.
Dear all,
After having used the Targetseq Exome Capture kit for years, LifeTech provided us with a new version of the kit called Ion... -
Nimblegen MHC SeqCap EZ
Is anyone doing HLA sequencing with the Nimblgene SeqCap EZ Human MHC design? Or are you doing HLA with your own custom pull-down kit? If so, I would... -
NimbleGen SeqCap EZ Choice Library-preparation for the PGM
Hello all.
From a previous project, we have a 'NimbleGen SeqCap EZ Choice Library'-design that we would now like to use for a new project... -
Capture or amplicon?
Hey all, I'm looking to sequence human exons across 189 genes totalling about 700kb. Is there anyone who's tried both target capture and amplicon sequencing... -
Sequencing DNA/RNA of 4 genes of interest in multiple samples (n=50)?
I am looking for ideas for sub-genomic sequencing experiments and I realy can use some help:
I would like to use at least 50 normal/tumor... -
Efficient targeted resequencing of human germline and cancer genomes by oligonucleoti
Hi everyone, check out our new sequence capture method, recently published in NBT. We are calling the method oligonucleotide-selective sequencing (OS-Seq)....Last edited by JBuenrostro; 10-26-2011, 09:30 PM.
