Hi everyone,
I'm using the ONT CRISPR-Cas9 targeted enrichment protocol (https://www.nature.com/articles/s41587-020-0407-5) to sequence a 18kb...
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AMPure XP clumps during Cas9 library prep
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Make your own libraries/barcodes?
Hello,
I am new to sequencing, and plan to perform genome-wide CRISPR/Cas9 screening as described in this paper - http://www.ncbi.nlm....
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Finding CRISPR gRNA off-target matches using NGS mappers
Hi all,
To keep it brief I have about 3 million, 23bp sequences (gRNAs) which are putative CRIPSR-Cas9 sites, all of which I need to screened...