8 results in 0.0021 seconds.
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Too many ZMWs failing after generating CCS from subreads
We sequenced a fungal genome using PacBio Sequel for a de novo assembly we want to perform. We received the subreads in BAM and FASTA format, as well...Last edited by salias; 05-08-2025, 03:18 AM. -
Create CSS with different adapters
Hi!
I've searched for a similar thread and wasn't able to find a solution so I hope that this question is not repeated.
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Is this a valid [CircularConsensus] Result Report?
Hi all,
I'm new to pacbio analysis. It's my first time to run SMRT analysis to get read of insert. I used the following command:
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PacBio palindromic amplicons
Hi,
We are trying to perform PacBio sequencing on 2-4 kb amplicons that have palindromic sequence. My understanding is that when building... -
Import raw PacBio data from *.bax.h5 files
Dear all,
I've been sent some raw PacBio data from a collaborator which I'd like to import into SMRT Portal to generate filtered subreads... -
Identifying heteroduplexes in PacBio CCS reads
Hello,
I'm working with circular consensus reads from a PCR product that may contain heteroduplexes. This is because the PCR was generated...Last edited by verheytb; 04-21-2015, 12:45 PM. -
Read quality calculation for a base in CCS
Hi guys,
I was wondering if anyone could tell me how PacBio calculates the quality scores while generating CCS sequences.
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how to filter CCS by number of passes (not by long read length)?
Hi, the default smrt analysis pipeline keeps the long reads that have at least 3 passes of CCS. For my project, I want to get >=6 passes.
I...
