Hi all,
I am making my first 10x single nuclei libraries and ran two chromium chips with 4 samples in each chip.
While...
35 results in 0.0043 seconds.
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help? cDNA variability
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NexteraXT kit on cDNA amplicon?
Can I use the Nextera XT library prep kit on a 2kb cDNA amplicon? Just wanting to check that the tagmentation process will work ok on cDNA. I've only... -
Reverse Transcriptase of choice for single cell cDNA synthesis
Superscript III, Maxima H-?
Its difficult to parse out the differences between many of the commercially-available M-MuLV RTs. Does anyone... -
Problems with cDNA 1st strand synthesis
Hi Seq peps!
I am hoping someone has done this before and knows the answer. I am using one of the targeted expression kits from Illumina... -
Is There Any Tools/Methods For PacBio IsoSeq New gene prediction analysis?
We have used the SMRT(Pacific Biosciences) sequencing system PacBio RS II to generate cDNA sequences. After isoform-level clustering, polishing and trimming... -
How should I compute the sampling depth or cells when I use pacbio RNA sequencing?
I want to do the RNA sequencing using pacbio SMRT, I have got the transcriptome information, but I don't know how many cells should I use ? And what the... -
Any Tools/Workflows For PacBio IsoSeq SSR Analysis?
We have used the SMRT(Pacific Biosciences) sequencing system PacBio RS II to generate cDNA sequences. After isoform-level clustering, polishing and trimming... -
After 1st-strand cDNA synthesis for RNAseq, is it safe to wait a day for 2nd?
Greetings All,
It's my first time doing library prep for RNAseq on a eukaryotic organism, and unfortunately I failed to order 2nd strand... -
GMAP problems: stopping at "Finished checking compiler assumptions"
Edit:
Never mind. It was a simple database error. Fixed it, but don't know how to delete post...
I am trying to map around 300 cDNA...Last edited by JonB; 04-28-2015, 08:03 AM. -
CDNA synthesis from FFPE: best kit/enzyme?
This is very much a beginner's question, but does anybody have experience with cDNA synthesis on RNA extracted from FFPE? We would like to ligated our... -
Is it possible to multiplex a cDNA and gDNA library together in one lane?
Hi
We are running a genomic library on a multiplexed miseq but we have space left for another library and were wondering if it is technically... -
How to annotate cDNA positions of intronic SNPs?
Hi all,
For my current research I'm dealing with big amounts of intronic SNPs.
Given a list of intronic SNPs with known... -
six frame translation
Hello everyone,
We have a RNAseq denovo assembly. From these cDNA sequences we want to get the protein sequences. How can i decide which frame... -
human cDNA lengths
Hi,
Does anybody have an idea where can I get the lengths (in bp) of all the human cDNAs?
Thanks
S -
Full-length cDNA and NGS
Hello everyone,
Is there such a thing such as full-length cDNA libraries that can be sequenced through NGS?
Thanks,
...
