Hi all,
i am wondering what are possible causes of low Q30 scores, apart from overclustering?
I am working with amplicon...
29 results in 0.0031 seconds.
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quality score issues
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specific compost/soil metagenomes causing underclustering of whole run?
For the last few sequencing runs we seem to have a strange problem with a specific set of soil/compost metagenome libraries. Whenever we add one of these...Last edited by jov14; 04-12-2022, 03:00 AM. -
Nanoarray sequenzing, cluster-plot
https://ibb.co/9qbR0st
I don't understand picture B, it`s an Cluster plot of normalized intensities from a high-density test array with 700nm... -
Large Library On Multiplexed Run
Hey all,
I'm currently running highly multiplexed runs on a Nextseq 550 and I've come into the problem where a library submitted to me... -
Biclustering methods
Hi everyone,
I am new to bioinformatics and new to this forum. Hope that I could learn more about bioinformatics here.
I... -
Cluster RNA sequences from fasta alignment by identity threshold
Hello everyone,
I just posted in biostars a post https://www.biostars.org/p/312195/ , maybe someone in here could help me or give any advice?...Last edited by December; 04-30-2018, 05:30 AM. -
Program for clustering rna seq reads using protein database
I have an RNA Seq data that I have used to assemble a de novo transcriptome, however, I am having trouble clustering it down into a final set of putative... -
scSeq Analysis - Seurat and OOBE usage
Hi all,
I have a specific stats question, that is a bit deep in the weeds (for me anyway, and also reveals some of my shortcomings, in... -
differences between alignment tools for single cell RNAseq data
I was wondering whether there were any major differences in the readout from single-cell RNAseq reads aligned with traditional short-read alignment tools...Last edited by jspagnuo; 11-17-2016, 01:01 AM. -
Clustering/Binning Metagenomic contigs with MyCC and 16S sequences
I have tried to use MyCC (http://www.nature.com/articles/srep24175) on samples with varied diversity and I always get very good accuracy between number... -
deseq2: analysis of time-series with 2 timepoints with control for only 1
Hi all
I need advice on how to analyse my complicated design RNAseq data with Deseq2.
I have 3 biological replicates, 2... -
Cluster complete plasmid sequences
Hey all,
I have complete nucleotide sequences for 119 plasmids, with sizes ranging from 500 to 170000 base pairs. Is there a way to cluster those... -
Clustering tool
Hello,
Could someone please suggest a free clustering tool that is similar to CLUTO (http://glaros.dtc.umn.edu/gkhome/views/cluto). I... -
HiSeq rapid run overclustering
Hello,
On the Illumina website, it says that the optical raw cluster density for PhiX runs is 850K-1000K clusters/mm2. But, what is the functional... -
Discriminant Analysis for RNAseq data
Dear all,
I am wondering if there is a good R package developed that can perform discriminant analysis for RNAseq data?
...
