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MiSeq Cycle 1 Possible Clustering Failure
We have run the same library on two different machines using a MiSeq V2 300 cycle kit and it has failed in cycle 1 both times. We are following the same...
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NEBNext adaptors clustering failed
Hi all,
I am having problems with the NEBnext Ultra kit for library preparation. I´ve been doing same amplicon libraries for three years with...
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Removing redundancy from GenBank plasmid database using cd-hit-est
Hey all,
I have the following problem. I have a plasmid sequence database (ftp://ftp.ncbi.nlm.nih.gov/genomes/refseq/plasmid/) that is...
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Miseq Error: No usable signal found in the images; it is possible that clustering has
Hello all,
I wanted to reach out to see if anyone has experienced this error, descript below. The run appears to have failed shortly after...