11 results in 0.0022 seconds.
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Can I use secondary antibody as my negative IP control in my ChIP seq experiment?
I am doing a ChIP experiment for H3K9me3 marks in HEK293T cells. We have the ChIP grade Ab for H3K9me3 but didn't order ab for isotype control. We have... -
Spiked-in control for microbiome
Hello! I am helping out with a microbiome project and my collaborators have had a problem that can best be explained by a sample naming error at the... -
Best control in RNAseq for homozygous mutant in transgenic background
What is the best control in RNAseq analysis for an homozygous mutant in transgenic background? (for zebrafish)
Should it be an heterozygous... -
Missing genotypes, in case control study using Plink
Hello,
I have a sequencing in ION PGM.
We sequenced 96 barcodes (individuals) and 310 amplicons (chromosomal regions).... -
ERCC ExFold vs. regular spike-in mix?
Does anyone know the practical difference between the Ambion ERCC RNA Spike-In Mix (Life Tech #4456740) and their ExFold mixes (#4456739)? The regular... -
How to subtract a coverage track of mock IP from a set of ChIP tracks?
I have a set of ChIP data, most of them with a transcription factor, but also control data, which was not the raw input chromatin, but instead they performed... -
Is it normal to hav very low mapping for TREATMENT file in Chip-seq?
Hi,
I'm working on chip-seq and it looks like I have an issue. I'm not sure if it's normal for a chip-seq analysis.
I have... -
Sequencing control for ChIP-Seq
Hello,
I have a question regarding sequescing control for ChIP-Seq:
In the chip we sequence only the regions of the DNA... -
Using DESeq to examine control variability
Dear all,
I have count data for 2 conditions, 10 normal samples (N) vs 10 diseased samples (D). When I compare for differential expression... -
Need Phix control sequence.txt
We just lost all our raw and archived Solexa data in a stupid SAN controller crash.
Not a scientific disaster, but I have a student... -
Control Lane - PhiX
Hi,
We have been sincerely using the control lane in all our solexa runs thus far. The auto5 option for Solexa pipeline phasing, when control...
