Hi,
I am currently analyzing a RNASeq experiments with 2 conditions. Each condition has several biological replicates and each replicate...
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DESeq2 merged vs unmerged replicates
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error in GenomicRanges package
hii everyone,
i was working with this package so i got some error
...Code:> library("GenomicFeatures") > testFile
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featureCounts segmentation fault
I have a lot of BAM files (from CCLE database), and I tried counting them using featureCounts.
Some of the files works great, but many(!) of them...
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DESeq2 design error
Hi,
I am trying to run DESeq2 using the "Differential analysis of count data ~ the DESeq2 package provided in the bioconductor website....
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Countdata from samtools idxstats
Hi
I have my .bam files sorted and indexed for my samples. Later I used the samtools idxstats to obtain the number of mapped and unmapped...
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Counting number of reads mapped to an allele
Hi,
I have a sam file and I want to count how many queries mapped to a specific reference in the reference file. Is there a simple way...
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Time-course analysis of sequencing count data
Hi,
I am trying to find information on time-course analysis of sequencing count data (eg, RNA-seq). I was told the latest version of edgeR...
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finding DE genes from VSD normalized data
Hi all,
I have time series mRNA-seq data but without replicates.
I have some doubts about calling DE genes with DESeq.
...
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error in DESeq analysis
Hello,
I have RNA-seq data, which I am trying to analyze with DESeq. My file (.csv) appears to be correct
>head(myfile)...