Hi,
It is explained in several places such as here
https://bioinformatics.cvr.ac.uk/ill...mer-sequences/
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T/A base from adapter cloning - part of sequencing read primer?
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Fully exonuclease-resistant custom oligonucleotides?
A phosphorothioate linkage between the last two nucleotides makes an oligonucleotide resistant to 3' exonucleases. The phosphorothioate bond is chiral,...
