Hi all -
I am planning an amplicon sequencing run on a NextSeq 550 that will require a custom read 1 sequencing primer. I am wondering if...
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NextSeq 550 custom read 1 primer GC clamp and Tm
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Custom primers for NextSeq: will they work on MiSeq and HiSeq?
I am wondering whether my experiment (which was originally designed to work on a NextSeq) will work on MiSeq and HiSeq.
It is a library... -
Spiking custom primers failed on Miseq runs
Hi all,
I would like to know if somebody encountered the same problem as we do at the moment.
Since Mid February we are no longer... -
MiSeq amplicon: corrected int spike
My last MiSeq 16S amplicon run (2x250) and a rerun with a lower cluster density both showed large spikes in corrected intensity (>1000) during the...Last edited by sharchaea; 05-26-2015, 08:51 AM. -
Has the NextSeq forgotten how to use custom primers?
Illumina Experiment Manager version 1.8.2:
Illumina Experiment Manager version 1.9.1 (current):
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Short LNA custom sequencing primer for MiSeq
Hi All,
For a couple of reasons we are trying to 'shorten' the custom sequencing primer we use in MiSeq. Primarily it is because we are trying...Last edited by bunce; 02-17-2014, 09:14 PM. -
Custom primers and PhiX on MiSeq
Hi all,
I have a question about using custom primers to sequence 16S rRNA using MiSeq. I have not done a run since last April and while reviewing... -
PCR Enrichment - Replace TruSeq PPC and PMM?!
Hi all, NGS newbie here. I know this has been discussed in other threads, but I don't really understand. I have already had the sample until ligation... -
Illumina Adapter and Primer preparation
Hi!
I have ordered the Illumina PE primers and PE adapters from IDT with the required modifications. I have combed through the forum, but... -
homemade primers and new kits
Has anyone had trouble using homemade (i.e. non-illumina) PCR primers over the past few months?
We use our own (unmodifed) oligos for PCR...
