Hi all,
I'd like to share a modular and transparent bash-based pipeline I’ve developed for pre-processing ddRADseq Illumina paired-end...
16 results in 0.0028 seconds.
-
Lightweight bash pipeline for ddRADseq read pre-processing, demultiplexing, and de-duplication using cutadapt
Last edited by rafalwoycicki; 04-23-2025, 06:10 AM. -
Calculate number of reads per sample for ddRADseq
Hi everyone,
I am planning a ddRADseq experiment with non-model plants (this means, no reference genome available), and I am wondering how... -
Calculate number of reads per sample for ddRADseq
Hi everyone,
I am planning a ddRADseq experiment with non-model plants (this means, no reference genome available), and I am wondering how... -
ddRAD raw read count difference by barcode
Hello,
We received the raw reads of our ddRAD library from a sequencing facility, and after demultiplexing, I noticed that there is a 10-fold... -
PCR-free ddRADseq?
Hi all.
I'm helping out on a RAD-seq project and we're exploring different options to assess population structure for several hundred... -
Spitballin': PCR-free ddRADseq?
Hi all.
I'm helping out on a RAD-seq project and we're exploring different options to assess population structure for several hundred... -
ddRADSeq Libraries
We seem to be having some difficulties creating reproducible ddRADSeq libraries.
We are noticing that after running the first of the processing... -
Problematic ddRAD library post-size selection bioanalyzer trace
Hi everyone,
I'm preparing a ddRAD-seq plate for Illumina HiSeq 4000 sequencing and I'd like to know if anyone has seen similar Bioanalyzer... -
ddRAD, STACKS, and Trimming
Please disregard/delete.
Last edited by Carcharodon; 07-13-2017, 04:51 PM. -
Alternatives to Pippin Prep for ddRAD?
My lab is interested in doing population connectivity/phylogeography on herps, and think that ddRAD is probably the best NGS option for us (since we don't... -
Pippin-Prep - External or Internal markers?
Hi Seqanswers community,
I going to test size selection step in Pippin-Prep and I´m not sure about the advantages and disadvantages to... -
How much phiX to spike in for ddRADseq library on HiSeq?
Our lab is preparing for a large scale ddRADseq project (~3000 samples). In doing the calculations for how many lanes of sequencing we will need to achieve... -
ddRAD library low concentration
Greetings,
I am making a ddRAD library and post-digest and cleanup my concentrations are very low. Since I have to standardize my DNA concentrations... -
Problem with uneven distribution of rads among individual libraries
Hello everyone!
I am totally new in NGS, so I apologize for the basic question.
I am doing ddRAD (EcoRI and SphI) for SNP analysis... -
RADSeq / Population Genomics with Polyploid Organisms
Hi all,
I am working with some polyploid organisms (ploidy not always known, but tetra and up) that we want to do some RADSeq / CRoPS sequencing...
