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Webinar: Metagenomic Sequencing and Analysis
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Question regarding Zygosity variant calling interpretation - homozygote variants
Hi everyone,
I have assembled the genome of a non-model organism - insect (de novo genome assembly) and performed read mapping to it. I... -
Min/Max allowable Ns in reads
Dear all,
How much Ns in a read should be allowed before discarding it? I have read a few studies removing reads with >1 N;... -
Minimum contig coverage to keep
Dear all,
I am working on de novo assembly of bacterial genomes. Genomes were sequenced with Miseq 2x250 libs with an average coverage... -
De novo small RNA assembly
Hello,
I want to de novo assembly small RNA reads generated from Illumina sequencing (final lengths after clipping and trimming are 18-32nt)....Last edited by Juulluu21; 06-23-2016, 06:56 PM. -
Ray Error: miscount of the number of reads
Dear all,
I am trying to run Ray to de novo assemble a nematode genome.
I run into the following error:
...Code:mpirun \
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Normalization / Transformation of DE data with a de novo assembly
Hello,
I have a question regarding transformation and/or normalization as it applies to analysis of differential expression (DE) for a...
