14 results in 0.0030 seconds.
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Demultiplexing IONA® generated data
I'm working on my MSc thesis (bioinformatic analysis of NIPT data) and I don't have access to reliable NIPT data, but recently I achieved some multiplexed... -
Split seq data based on index AND barcode
Dear all,
I am currently designing an experiment, where my samples will have both a barcode and an index.
Barcode: Sample-specific...Last edited by Meyana; 01-26-2020, 08:25 PM. -
Post-demultiplex adaptor removal?
If one has demultiplexed dual index reads on a MiSeq AND included "adaptor removal" as part of the demultiplexing (on instrument), should one... -
Discrepancies in Demultiplexing programs - help request
I have Illumina PE data from a MiSeq run and am finding large discrepancies in the number of reads recovered while demultiplexing.
Originally,... -
Large Discrepancies in Different De-multiplexing programs - help request
Hello,
I am working with Illumina PE reads from a MiSeq run and am having trouble correctly de-multiplexing them.
The fastq files... -
Issue with FASTA header in QIIME
Dear all,
I have to analyze a set of 26 samples of 16S amplicon data, coming from 250 nt Paired-end Illumina Hi-Seq reads. When I received... -
Demultiplexing disabling FastQC
Hi all,
I got a big file of data back from the sequencing centre that worked fine when I put it through fastqc, but after demultiplexing... -
inline barcodes appearing in reverse read of pair
Hi All,
I have Illumina 101bp paired-end data where the libraries were prepared with custom inline barcodes at the P5 end. So the first... -
Flowcell with single and dual indexing demultiplexing issue
Hello,
I have received from my lab one HiSeq flowcell with 7 lanes single indexed and the 8th is dual indexed.
Something... -
Demultiplexing 454
Dear all,
I am trying to de-multiplex a 454 fasta file.
It is a public data file. In the paper (PMID:21731642) the authors say... -
Demultiplexing a MiSeq library based on Amplicon Sequence divergence?
Hi All,
My lab works on a lot of diverse taxa. We want to sequence the mtDNA genomes of each using our MiSeq. We generated long-range PCR... -
454 problem with adapter trimming and MIDs
We've had some odd trimming results and I thought I'd check whether anybody else has had a similar experience.
I received raw 454 reads... -
using Mothur to demultiplex plate prepared using RLMIDs
Hi,
I am trying to switch over from the Roche tools (sfffile, sffinfo..) to using Mothur to demultiplex some metagenomics data. However one plate... -
How to Demultiplex a Nextera paired-end MiSeq run
Has anybody been able to successfully demultiplexed a Nextera paired-end MiSeq run?
The current MiSeq Reporter cannot demultiplex and produce individual...
