Hi,
I am trying to switch over from the Roche tools (sfffile, sffinfo..) to using Mothur to demultiplex some metagenomics data. However one plate was done using RLMIDs
I am not sure as to how to set up the oligo file in such a case to process the data.
There seems to be two barcode sequences perline as opossed to a single barcode
in the MIDconfig.parse file:
mid = "RL1", "ACACGACGACT", 2, "AGTCGTGGTGT";
....
I am wondering what the two barcodes represent and whether it is possible to set up
Mothur to process this plate.
Thanks!
I am trying to switch over from the Roche tools (sfffile, sffinfo..) to using Mothur to demultiplex some metagenomics data. However one plate was done using RLMIDs
I am not sure as to how to set up the oligo file in such a case to process the data.
There seems to be two barcode sequences perline as opossed to a single barcode
in the MIDconfig.parse file:
mid = "RL1", "ACACGACGACT", 2, "AGTCGTGGTGT";
....
I am wondering what the two barcodes represent and whether it is possible to set up
Mothur to process this plate.
Thanks!
Comment