Hello,
I have a data of snatacSeq of 15 batch of patients (5 patients per batch that are multiplexed ), I need to demultplexe them using genotypes...
28 results in 0.0062 seconds.
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Lightweight bash pipeline for ddRADseq read pre-processing, demultiplexing, and de-duplication using cutadapt
Hi all,
I'd like to share a modular and transparent bash-based pipeline I’ve developed for pre-processing ddRADseq Illumina paired-end...Last edited by rafalwoycicki; 04-23-2025, 06:10 AM. -
Cutadapt Demultiplex
Hi all,
I'm working in R and trying to demultiplex some data using cutadapt
I have three files:barcodes.fastq.gz (which I have... -
Dual-indexed library: String of G's for 1 of the 2 Indexes - Adapter Dimers?
A dual-indexed library was sequenced on the Illumina iSeq100.
When looking at the most popular index pairs within the DemultiplexSummaryF1L1.txt... -
demultiplex BCL data with two different barcoding systems
Hi,
I was wondering if anyone has experience in demultiplexing BCL files from Illumina with two different barcoding systems in one go?... -
Demultiplexing failures in MiSeq run
Hi All!
I am sequencing (on a MySeq platform) a custom library containing 4 i7 indexes. In principle, it works, but when I look on demultiplexing... -
demultiplexing without clocs files
Hi,
I have an Hiseq run in which the colcs files were deleted, but I still have the rest of the data. I also have a folder (~160GB, much more then... -
Demultiplex nanopore reads with custom barcodes
We have a problem trying to demultiplex MinION sequences with custom barcodes. Do you have any software recommendations we can try for demultiplexing... -
add UMI sequences to fastq read name
Dear all,
I have paired-end fastq data generated with Illumina bcl2fastqv2.19 & sequenced on a Novaseq.The i5index is 7bp long, the... -
demultiplexing multiple runs with and without samples same sample sheet
Hello all,
I'm going to demultiplex a run with a sample sheet in which there are entries and adapter sequences in the sheet with no corresponding... -
Advice needed for --use-bases-mask option (bcl2fastq)
Hi all,
A colleague in the lab asked me to demultiplex a recent NextSeq run. She loaded it with samples prepared from two libraries. One... -
Demultiplexing PacBio sequences
Hi all,
I have the following problem:
I have a dataset containing circurlarized and linear DNA. It was amplified with two pairs of primers... -
demultiplex FastQ files based on read names
Hi everybody,
my name is Fabian Roger and I am a PhD student in Ecology at the university of Gothenburg.
I am at a stage... -
demultiplexing dual index Illumina reads with mismatch tolerance
I'm analysing genotyping-by-sequencing data for 2,100 dual-indexed samples and am looking for a fast, mismatch-tolerant demultiplexer.
... -
generate fastq files for entire sample pool, remove human reads, then demultiplex
I have 125xPE Hiseq data with dual Nextera indexes (each 8 bp). My data is from a single lane of the flowcell, but I have my lane's .bcl and .cpos files....Last edited by sk8bro; 10-29-2015, 09:30 AM.
