148 results in 0.0032 seconds.
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Effect of Over-Represented Transcripts on DEG Analysis
We have a fairly simple set of RNA-seq data that we are using to perform a DEG analysis, breifly, a wild-type strain and a single gene knock out (in yeast).... -
Different DESeq2 results (both FC and pvalue) by changing col/row orders in input
I'm getting different p-values and FCs from DESeq2 (v1.34.0) by simply changing the order of the columns in the count table. I can't understand why. I... -
Different DESeq2 results (both FC and pvalue) by changing col/row orders in input
I'm getting different p-values and FCs from DESeq2 by simply changing the order of the columns in the count table. I can't understand why. I made sure...Last edited by metheuse; 06-20-2022, 08:23 AM. -
Basemean threshold
I have an rna seq dataset and I am using Deseq2 to find differentially expressed genes between the two groups. However, I also want to remove genes in... -
Differential Expression analyses between species
Hi all,
The project I am currently working on involves five closely related cricket species in the same genus. I have 8 (neutral) replicates... -
Deseq2 normalization between and among the samples
Hello everyone,
I would like to know how the normalization of DeSeq2 works among the replicates of the same sample and between different treatment... -
End-to-End Bulk RNAseq Analysis on Demand
Hi everyone,
Really excited to be a part of this community. I am one of the team members of BioBox Analytics. We're a group of former... -
DESeq2 model design + contrasts (not full rank)
delete ditLast edited by CAGCAGCAGCAG; 06-19-2020, 01:02 AM. -
DESeq2 model design + contrasts (not full rank)
Hi,
I'm new to this forum and I got a question doe DESeq2 concerning model design and contrast, because my model design is not full ranked.... -
DESeq2 interaction term results differ between full dataset & subset
Hi all, hoping someone has some insight on this because I am completely stumped...
I'm using DESeq2 for differential analysis... -
How do I differentiate outliers from in-group variations in a DESeq2 PCA plot?
I have a PCA plot (please see below) from DESeq2'sfunction. I have 9 samples distributed in to...HTML Code:plotPCA(vsd, intgroup=c("conditions")) -
Loss of relevant genes in NGS...
Dear SEQanswers community,
I have a quite fundamental concerns regarding using NGS to explore differentially expressed genes.
I... -
DESeq2: Contrasts in multifactor design with interaction terms
Hello,
Please note that I have posted this question on another website, but I did not receive any advice. I would really appreciate some... -
understanding design formula in DESeq2
Hi,
I am trying to understand the role of using an interaction term in the design formula of DESeq2. I have read this explanation: ht... -
DESeq2: model design in rna seq - person variability
I have 8 samples that correspond to 4 persons measured in two times, 0h and 20h.
names_chip person time Dample
1 IonCode_0109 ...
