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SEQadmin2 started a topic International Team Sequences the Genomes of Indigenous Peoples Across the Americasin NewsInternational Team Sequences the Genomes of Indigenous Peoples Across the Americas
An international research team has sequenced the largest genomic dataset yet assembled for Indigenous peoples of the Americas, generating 199 genomes... -
Reduce PhiX
Dear all,
I was wondering if there was any tool to help me reduce the PhiX% used in our NextSeq 500 runs? We run similar samples continuously,... -
PCoA Plots
Hi Everyone,
I have HiSequencing data that I want to set up PCoA plots for.
These are environmental samples and I want to look at the diversity... -
RNA-seq: Population diversity for a gene based on normalized counts?
Hi all,
A little Bioinformatics/Population Genetics question. I have a list of significant differentially expressed genes from two experimental... -
Comparing nucleotide diversity between two paralogs using a VCF file
I'm looking at two homeologs and trying to gain information about their potentially different evolutionary rates. There is a publicly available dataset... -
MiSeq - minimum index diversity?
Hi, we want to do a MiSeq run of a library pool of TruSeq DNA PCR-free (single index; 6 bp), and custom 16s amplicons (dual index 8+8 bp). We wish the... -
OTU picking
Aloha,
I would like to get your input on how and when in the analysis process you pick your OTUs. Let's say you have 14 different samples... -
Long amplicons failed to sequence in MiSeq run (where shorter random DNA succeeded)
Hi SEQanswers,
Our third MiSeq running trying our various protocol incarnations has failed, just wondered if anyone might have any ideas... -
best 16s region to amplify with NGS ?
Does someone have an idea of the best 16s gene region to amplify with NGS in order to assess microbial (bacterial) diversity in environmental samples... -
RTA v2.8 : Conflicts with low complexity sequence
We routinely use an in-line barcoding system with bases 1-4 as variable barcode nucleotides and base 5 is a mandatory T in all indexed libraries.
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