Hello everyone,
I prepared a custom library using dual index TruSeq adapters for a Miseq run with a sample plexity of 3. This is the first...
7 results in 0.0023 seconds.
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Why do some index sequences have an incorrect base?
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index diversity on MiSeq
Can I run 2-3 index combinations on MiSeq? I'd be using Nextera XT DNA sample preparation index kit and MiSeq v2 reagent kit. If I select index pairs... -
demultiplexing dual index Illumina reads with mismatch tolerance
I'm analysing genotyping-by-sequencing data for 2,100 dual-indexed samples and am looking for a fast, mismatch-tolerant demultiplexer.
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demultiplexing with dual indexing
Hi all, I am trying ascertain that my libraries can be demultiplexed. What we have are 6 samples prepared with the Nextera XT kit (whole genome shotgun).... -
How to perform this experiment.
Hi to all. Unfortunately I still without understand completely the NGS technology. So, I need that someone give me some tips or workflow to carry out... -
How to mix single index and dual index TruSeq libraries
In principle I see no issues with mixing dual and single index TruSeq libraries in the same run. The result will be that for the i7 read, the normal single-index... -
Mixing dual and single index TruSeq samples in a single MiSeq run
Should be possible, right? What happens if you specify both in a sample sheet?
I presume that if you specify a mixture of 6 and 8 base...
